ecogenics3:

outside temperatures of 20F and up, with geothermal water temperatures at 56f heated to 65-75f (a narrow   delta T  range) we have maintained   algae blooms and fish propagation in the winter and  ambient air temps within the  system at seventy five to eightyfive F

also, i have always claimed ten pounds(wet) per day algae  production rates but just looking at the winter-summer production DVD one can see clearly that production rates are far greater than that...

 but we are very conservative in our claims and just look what kind of  criticism we get from  people like you. these DVDs should put all the naysayers squabbling to rest once in for all unless of course, people wont beleive thier eyes either...

10lbs/day wet x 75% water = 2.5lbs of algae.  Lets say the BTU = 10000BTU/lb. At even a generous rate of 35% conversion, that means you have grossed 2.5KWH's worth of energy. Given a GeoThermal conversion of +400%, that gives you 10KWH's worth of BTU yield a day or 34130BTU/day. The definition of a BTU is that 1 BTU = 1 lb of water raised 1F. This means you grow enough algae that you can raise 34130 lbs (4112 gallons) of water 1F. How big is your system again? And where is all the energy to raise the temps a delta of 50?

By the way... a very small % of overall heat leaves side walls because heat rises. And very few people pump outside air into their 2x poly layers. 2x poly layer air blown has roughly a R 3 value. 

you know what froggy, throw all the F'ing numbers around you want   our system works and we have the proof  in the vidios  over many years of winter performance.

.you are incorrigable  and you resemble the hole that is situated nether parts of a derreire .im not even going to dignify your bullshit any more you have hijacked my forums often enough you may be able to spout theory  but you have no idea about  the way things work in the field.

bessez moi mon derriere cochon

Marc, I want to come see your system in person.  How do I arrange it?

I am !not! a potential investor or customer.  But I do know a few of both; and I have some government contacts that might be useful if your stuff looks right.

voltaire,

we are not open to the public at this time since we have a series of seminars under contract back to back untill late may when we will be holding yet another  seminar that will be open to the public,.around that time and throughout the rest of the  year well be glad to have you visit. if you wish. however.youll have to give us at least two weeks notice since we are   going to be to working on various projects in other states building algae production facilities for clients and our partners. in the meantime, Ive talked  to my webmaster and hes going to  try  to put a brief excerpt of the winter and summer  performance vidio on our website for all to see..

Marc

What is your estimate as to how much algoil you will produce in 2008?  If I wanted to, could I buy algoil from you?  If I could buy algoil from you, how much could you provide?  And at what prices?

These are not just idle questions.  I've got community and state organizations sniffing around for sources for just about every maturity step in the biodiesel production process.

The vast majority of them simply do not have at present the expertise to make their own algoil.  They are going to need to bootstrap their way up a knowledge and infrastructure curve in an economically sane manner.  These folks need products +and+ knowledge.  If Froggy wasn't such a naysaying @ss about algoil biodiesel, I could probably find him some lucrative consulting work. 

 VOLTAIRE,

at this time we have not decided how much algaeoil we will produce to sell,

 I beleive we will make biodiesel from it instead.

 also one of the largest biodiesel producers in the U.S. has expressed interest through a letter of intent to purchase algae cake from us.

 I published production figures and the economics of value added products  of our closed loop ecosystem on previous posts based upon individual units and acre sized complexes so i wont repeat them here. but in will try to find them and referr them to you.

 one of the most important factors that will decide which way we go is if we succeed in purchasing outright the facility in georgia which has pondage totaling200 million 800 thousand gallons. of infrastructure already buily on forty acres of land. theres a couple of pix on the pix section of this  forum showing just a little of the georgia facility. I cant even begin to calculate the amount of biodifuels or algae oil we could produce there.... it boggles the imagination..

 we are in negotiations with a company that is interested in partnering with us on that tremendous venture. if our negotiations come to fruition this will undoubtably be the largest algae production facility in the united states

 our potential partners are extremely excited about this possibility.and im impressed by thier enthusiasm and thier proactivity so this will make new possibilities come to the forefront

so if this becomes reality (LOL) it will certainly color the way our future ventures evolve.

no matter what ,Ecogenics will always remain a research and educational facility and a new entity will evolve independantly from it to deal with this mind boggling venture which we have been working on for a while now.

in the meantime our seminars will go on as scheduled and our r&d work here will continue as always.

marc

You may make things even easier for me than I expected.

I have multiple communities and some state agencies asking me about biodiesel.  Some of them are =very= willing to buy instead of locally produce.  They are only considering local production because they think they have no choice at the volumes they think they will need.

If a community was willing to sign a PO for a firm commitment for a specific number of gals of biodiesel per month, could you guarantee supply?  The numbers involved are ~100K gals per month on the low end and ~2M gals per month on the high end.  There are multiple contracts of this type to be had if someone can guarantee supply. 

If you can supply on that scale, at what prices?

ATM, my target is ~$5 US per gal Retail for heating season 2009.

voltaire,

 contact me off list at my email adress ecogenics3@aol.com we can continue discussing this between us...

 Marc

ecogenics3:

voltaire,

 contact me off list at my email adress ecogenics3@aol.com we can continue discussing this between us...

 Marc

It probably will not be me,  but I will have someone get in touch with you.

INSTRUCTIONS FOR EXPANDING YOU  ALGAE CULTURE

Take the 50 ML culture you recieve and add it to 200ml.of properly prepared medium (fresh or marine)over which you have a 25 or 30 watt cool white floursecent light,also, you should have an aereation pump hose and airstone going at all times to prevent stratification and keep the algae in suspension,average temp 75 to 80 max, Once the 200 ml culture has "greened" add another 200 ml of medium solution once that has also greened keep repeating this process untill you have a litre of greened culture.once you have a litre you can add the litre to a gallon of medium and from there to ten gallons and from ten gallons to 1000 and above gallons. ten gallons will easily seed up to 4000 gallons. and more. be patient depending on which algae you choose, the rate of growth will vary, favorite algae seem to be Tetraselmis and Chlorella minutissima Judging from the amount of orders we get.in previous posting on this lipid content thread we have listed the algaes in our collection and thier lipid content. we have received many requests for the  above instructions, please be courteous and dont hijack this posting as you do a disservice to those who want to learn how to grow algae. stay on topic please...you will also need one gallon glass jars, and ten gallon aquarium tanks ( available at walmart) an aereation pump, tubing and air stone, and instant ocean aquarium salts for marine cultures.

hope this is helpfull

 Marc

 What a waste of time . I sure lerned a lot .

froggy and ecogenice3 were perticulary intreasting 

there are yeasts fungi and molds that contain fats also that should be explored..

 YEASTS                      fat content

candida  lipolytica              36 %

trichosporum  cutaneum     45%

candida curvata                 58%

lipomycens lipferus           63%

MOLDS                     fat content

eutomophtora virulenta    26%

aspergillus flavus            28%

pytium ultimum              49%

fusarium bulbigenum       50 %

these can be genetically optimised to produce oils using inexpensive biomass substrates such as crop residues, wood wastes, MSW biomass.

we must explore all avenues of biooil production these are even easier to grow and can be grown in a true closed loop as the residues from extraction may be recycled back into the production system which can be in a indoor manufacturing scenario.

combining these with algae production can produce a double whammy of oil production resources. we will be expanding our laboratory to explore these possibilities for thier potential

 Marc

here are a few more  yeasts and molds that have great potential. for biooils

YEASTS

endomyces vernalis 65%

rhodotorula glutinis 71%

MOLDS

      aspergillis fischeri 53%

   penicilium lilacinum 56%

mucor circinecelloides 65%

unlike algae these dont require large amounts of water and could be grown in abandoned mines and buildings without much infrasructure

marc

Froggy and Ecogenic, Get  room!

This lovers quarel has gone on long enough.

Your both full of algae! 

Arthrobacter AK 19 is a soil bacterium that can produce up to 78.3 % of its  dry cell  matter as oil. the oils are very simil;ar to other vegetable oils. these fat producing organisms are grown  in a carbohydrate rich environment, the  production of fats is induced by nutrient deprivation .. these microorganisms are low cost feedstocks for oil production and require  very little land or water some estimates state that these organisms may be made available for 2.5 cents per pound and may produce a pound of oil for as little as 10 cents per  pound (feedstock cost). we envision a mix of production systems growing both algae and selected yeasts, bacteria, fungi and molds... those selected and genetically manipulated to maximise yield..

 By"hedging the bet" and considering polyculture as well as  factoring in a cascading stream of value added products,investment risks can be reduced and successfull outcomes are more likely.

In addition to  algae culture ,we will soon be  exploring the potential of  these organisms to contribute to the  production of future fuels. that can replace fossil fuels successfully. and in short order.

Marc

Brought this back up, I have gone over several pages worth of reading throughout the months I have become interested in doing this....

So, I am increasing more curious how I would be able to obtain a tetraselmis chui sample, as it sounds to be a faster growing strain, and as an individual gardner myself, am anxious to try and experiment with it on my own...... Would anyone be able to point me to a source???

Man, I have a lot to look forward to, just stratched the surface, gimme time to read over everything, too!!

Thanks

Brad

Yeah, you want to measure what nutrients your algae are taking up. but perhaps this information already exists? There's probably some refernce material out there so you won't have to spend the $400 (froggy's quote) for (chromatography?). The problem is, what happens when conditions vary? Do you think the reference materials are going to cover all possible conditions? certainly not! So my guess is to try to find out from research materials where you should start, and then once things are moving along or there's some good reason to vary from the conditions quoted, then spend the $400.

If you're impatient and just want to grow some algae for a high-school science project or whatever, then soil-water sounds like a good shortcut. Not sure why you have to boil it more than once, though. 

 I've been building a digital library (including a section on phycology) for a while now, and encourage others to do the same. I've found a local university (Tufts) that has one of the books I need to check on the shelf. I am going there to photocopy a few pages. Perhaps I'll share, if other people make an effort to disclose information they've been able to dig up (Thanks for posting the numbers that you did, Marc).

 Marc vs. froggy though, I am getting tired of reading the vitriol. With the quotes you both make about your experience, you seem as though you are professionals in the field. Please respect each other like professionals do, and debate each others findings with detached dignity. I am not saying that i haven't seen FAR worse on the Internet, but you should both remember the maxim:

 'Never debate with an idiot; sometimes bystander's can't tell the difference"

Bob,

The reason for boiling twice is to insure that you purify the soil-water.  So that you do not contaminate your culture.  If you are trying to produce valuable results, you need a pure strain of algae and to keep it pure (not just "grow some algae", but grow a specific strain).

hello marc

i was just wondering if you would mind expanding on this a little please?

have you already started researching molds and yeasts and from your own research you obtain these lipid values, or were you able to find the info on the net elsewhere?  how do/did you intend to harvest the oil from these sources, or is that still in the thinking stage?

thanks and best regards

hi,

 were still setting up the  fungus and mold lab,

those production numbers were gleaned from various technical papers

 we plan on finding out first hand if those prove out. evrything is on hold here untill may as we are preparing for our TIES seminar next monday and tuesday

.after that important event, well get the fungus and mold lab up and running and well be offering another "how to grow algae seminar" to the general public in mid may.

from what I have understood ,the fungus and mold offer great promise for biofuels production, there are some abandoned coal mines that might be available to grow these not too far from us.well do some preliminary study here at our lab first to determine viability.

 Marc