Nesna,
DI water is de-ionized water - the "ulitmate" distilled water.
All
of the nutients needed to grow anything are available as
chemicals. See this site for one particular formulae for some
species of algae:
http://www-cyanosite.bio.purdue.edu/media/table/f2.html
For instance, notice that they include the iron the algae needs as
FeCl3·6H2O.
It
will probably be possible to grow algae with manures, organic wastes,
etc. Today we do not know enough about what we are doing to be
able to know how to feed the algae. To be able to design
reproducible experiments, we need to start with known strenghts of
chemicals. We can get the chemicals in a known strength, so when
we find out how much to use, we have a solid fix on what we were
feeding the algae. If you haphazardly feed some rotting garbage
to the aglae, you will have no idea of what you fed the algae.
Even if you tell me exactly what you did, I would not be able to
reproduce your results. My rotten tomatoes may only be half as
big as your rotten tomatoes. Yours may have a fungus growing on
them that produces B-12 while mine doesn't have that fungus.
(Some speices of algae require B-12.)
So, at this stage in our
knowledge about growing algae, we need to learn how much to feed the
algae to get maximum growth. After we learn more about growing
algae, we will be able to figure out how to supply the needed nutrients
from organic sources.
For our experiments at this stage, we are not too concerned about costs.
When
we get to production, I hope we will be able to use manure, so the
costs will be minimual. If you need some numbers, the report of
the ASP has cost estimates for everything, including chemical fertilizers.
There are two source
for CO2. The atmosphere is my preference. Some people look
to get it from another process such as flue gasses or ethanol
fermentation. PBRs will probably require pumping the CO2 into the
culture. Raceway ponds can use pumps if the CO2 come from other
processes or paddlweheels if the atmosphere is the source.
As discussed above, pass the garbage along to the garbage collector.